免疫相关基因表达与惊恐障碍的关联研究

Research on the association between immune-related gene expression and panic disorder

中文关键词:  惊恐障碍  基因表达  惊恐障碍严重程度量表  免疫

英文关键词:Panic disorder  Gene expression  Panic disorder severity scale  Immune

中文摘要:

      背景 遗传因素在惊恐障碍的发病机制中起重要作用，既往研究显示，免疫系统失调与惊恐障碍等精神疾病密切相关。然而，免疫系统相关基因表达与惊恐障碍的关系尚不清楚。目的 探讨CXCL8、IL6R、JUN、PTGS2、TGFBR1、TLR2、CCR4基因表达与惊恐障碍的关系，为惊恐障碍的诊断和治疗提供参考。方法 纳入2020年1月—2021年3月在四川省人民医院心身医学中心门诊及住院部就诊的、符合《精神障碍诊断与统计手册（第4版）》（DSM-Ⅳ）惊恐障碍诊断标准的患者作为研究组（n=52）。同期在成都市招募与研究组年龄和性别相匹配的健康人为对照组（n=72）。使用惊恐障碍严重程度量表（PDSS）评定惊恐障碍患者的症状严重程度。采用实时荧光定量PCR对研究组和对照组进行基因表达水平检测。采用Spearman相关分析考查研究组免疫相关基因表达与PDSS评分的相关性。结果 惊恐障碍患者JUN、PTGS2和TGFBR1基因的表达均高于对照组，差异均有统计学意义（Z=-4.172、-2.086、-3.018，P<0.05或0.01），经FDR多重校正后，两组JUN基因和TGFBR1基因表达比较，差异仍有统计学意义（P均<0.05）；两组CCR4、CXCL8、IL6R、TLR2基因表达比较，差异均无统计学意义（P均>0.05）。相关分析结果显示，惊恐障碍患者JUN基因表达与PDSS评分呈正相关（r=0.360，P<0.01），而CCR4、CXCL8、IL6R、PTGS2、TGFBR1、TLR2基因与PDSS评分的相关性无统计学意义（P均>0.05）。结论 JUN基因和TGFBR1基因表达可能与惊恐障碍存在关联，JUN基因表达与惊恐障碍严重程度有关。

英文摘要:

      Background Genetic factor plays an important role in the pathogenesis of panic disorder. Previous studies have revealed that immune system dysregulation is closely related to mental disorders such as panic disorder， while the relationship between panic disorder and immune-related gene expression remains unclear.Objective To explore the relationship between the expression of CXCL8， IL6R， JUN， PTGS2， TGFBR1， TLR2， CCR4 genes and panic disorder， providing references for the diagnosis and treatment of panic disorder.Methods A total of 52 patients who met the diagnostic criteria for panic disorder according to the Diagnosed and Statistical Manual of Mental Disorders， fourth edition （DSM-IV） were enrolled at the Psychosomatic Medicine Center of Sichuan Provincial People's Hospital from January 2020 to March 2021. Another 72 healthy individuals matched for age and gender from Chengdu were concurrently recruited as control group. The Panic Disorder Severity Scale （PDSS） was used to assess the severity of symptoms in panic disorder patients. Quantitative real-time polymerase chain reaction （PCR） was used to detect gene expression levels in two groups. Spearman correlation analysis was adopted to determine the correlation between PDSS score and immune-related gene expression in research group.Results The expression of the JUN， PTGS2 and TGFBR1 genes were significant higher in panic disorder patients than those in control group （Z=-4.172， -2.086， -3.018， P<0.05 or 0.01）. After false discovery rate （FDR） correction for multiple testing， the differential expression of JUN and TGFBR1 genes remained statistically significant between two groups （P<0.05）. There was no significant difference in the expression of CCR4， CXCL8， IL6R and TLR2 genes between two groups （P>0.05）. Correlation analysis revealed that the expression of the JUN gene in panic disorder patients was positively correlated with PDSS score （r=0.360， P<0.01）， while the CCR4， CXCL8， IL6R， PTGS2， TGFBR1 and TLR2 genes showed no statistically significant correlation with the PDSS score （P>0.05）.Conclusion The expression of the JUN and TGFBR1 genes may be associated with panic disorder， and the expression of the JUN gene correlated with the severity of panic disorder. ［Funded by Science and Technology Plan Project of Sichuan Provincial Department of Science and Technology （number， 2021YJ0440）］